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stmn2 gfp  (OriGene)


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    Structured Review

    OriGene stmn2 gfp
    Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or <t>Stmn2-GFP,</t> or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.
    Stmn2 Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stmn2+gfp/Stmn2+(NM_025285)+Mouse+Tagged+ORF+Clone/bio_rxiv__64898__2026__04__02__715646-35-0-10
    Average 94 stars, based on 1 article reviews
    stmn2 gfp - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells"

    Article Title: Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells

    Journal: bioRxiv

    doi: 10.64898/2026.04.02.715646

    Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or Stmn2-GFP, or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.
    Figure Legend Snippet: Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or Stmn2-GFP, or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.

    Techniques Used: Transfection, Plasmid Preparation, Cotransfection, Negative Control, Over Expression, Immunofluorescence, Fluorescence, Knockdown

    Related Articles

    Plasmid Preparation:

    Article Title: Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells
    Article Snippet: LAMP1-RFP was a gift from Walther Mothes (Addgene plasmid#1817; http://n2t.net/addgene:1817;RRID:Addgene_1817 ). .. Stmn2-GFP was obtained as the Stmn2 mouse tagged ORF clone (OriGene, cat#NM_025285) and pcDNA3-eGFP was used as the vector control. ..

    Control:

    Article Title: Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells
    Article Snippet: LAMP1-RFP was a gift from Walther Mothes (Addgene plasmid#1817; http://n2t.net/addgene:1817;RRID:Addgene_1817 ). .. Stmn2-GFP was obtained as the Stmn2 mouse tagged ORF clone (OriGene, cat#NM_025285) and pcDNA3-eGFP was used as the vector control. ..



    Similar Products

    stmn2 gfp  (OriGene)
    94
    OriGene stmn2 gfp
    Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or <t>Stmn2-GFP,</t> or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.
    Stmn2 Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stmn2+gfp/Stmn2+(NM_025285)+Mouse+Tagged+ORF+Clone/bio_rxiv__64898__2026__04__02__715646-35-0-10
    Average 94 stars, based on 1 article reviews
    stmn2 gfp - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or Stmn2-GFP, or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.

    Journal: bioRxiv

    Article Title: Stathmin-2 Mediates Paracrine Hormone Regulation of Glucagon Through Lysosomal Trafficking in αTC1-6 cells

    doi: 10.64898/2026.04.02.715646

    Figure Lengend Snippet: Cells (n=3) were co-transfected with LAMP1-RFP and GFP vector or Stmn2-GFP, or with scrambled siRNA or siRNA against stathmin-2 (Stmn2-KD) and live images were captured 24 hours post-transfection. a) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with GFP alone as a negative control (upper panel). After co-transfection with Stmn2-GFP, (lower panel), LAMP1-RFP appears exclusively in the intracellular region (yellow arrowheads). b) LAMP1-RFP is present in the cell periphery and the intracellular region (yellow arrowheads) after co-transfection with scrambled siRNA sequences as a negative control for Stmn2-KD (upper panel). Transfection with Stmn2 siRNAs (KD) resulted in LAMP1-RFP exclusively in the cell periphery (yellow arrowheads) (lower panel). c) Transcription factor EB (TFEB) is not translocated to the nucleus upon overexpression of Stmn2-GFP. Immunofluorescence images of fixed cells show the subcellular distribution of TFEB, GFP alone (upper panel), Stmn2-GFP (lower panel) and cell nuclei (DAPI). Box plots show the ratio of nuclear to cytoplasmic TFEB immunofluorescence. Values were expressed as the average nuclear/cytoplasmic TFEB intensity ±SEM (n=4). Data points in colour represent the average ratio of 8-16 cells per coverslip; black points represent ratios from individual cells. d) Stmn2 regulates the lysosomal transport protein Arl8. Immunofluorescence images of fixed cells show the presence of Arl8 (orange) in cells transfected with scrambled siRNA or siRNA against Stmn2. Quantification of fluorescence (left) shows that knockdown of Stmn2 (Stmn2-KD) significantly increased the fluorescence intensity of Arl8. Values are means ±SEM (n=6). Data points in colour represent the average fluorescence intensities in 8-16 cells per coverslip; black points represent values from individual cells.

    Article Snippet: Stmn2-GFP was obtained as the Stmn2 mouse tagged ORF clone (OriGene, cat#NM_025285) and pcDNA3-eGFP was used as the vector control.

    Techniques: Transfection, Plasmid Preparation, Cotransfection, Negative Control, Over Expression, Immunofluorescence, Fluorescence, Knockdown